Fermentation of Plant Extracts Supplemented with Milk Components by Lactic Acid Bacteria Produces Soluble Agonists for Toll-like Receptor 2 Possibly Suitable for Cosmetics

Stimulation of Toll-like receptor 2 (TLR2) on epidermal keratinocytes results in the tightening cell–cell junctions between keratinocytes; therefore, appropriate agonists for TLR2 could be promising ingredients cosmetics. However, a method to produce significant amounts soluble using materials that are suitable preparing cosmetics has not yet been developed. In this study, we tried identify lactic acid bacterial strains and media fermentation obtain from traditional fermented foods natural food sources. We found Lactobacillus delbrueckii subsp. lactis TL24 (TL24) combination hot water extracts asparagus edible stem cow skimmed milk were best strain culture medium, respectively, purpose. The ferments effectively stimulated HEK293 reporter cells expressing human their surface also inhibited paracellular molecular transfer cell sheet primary keratinocytes. Since these effects suppressed by anti-TLR2 neutralizing antibodies, it is proposed elicit via TLR2. Taken together, suggest containing potential